Fine Tuning of Real Time PCR as a First Tool for the Detection of G143A Substitution in <i>Venturia inaequalis</i> Samples

Apple scab caused by Venturia inaequalis (Cke.) Wint. is the most important disease of apple trees worldwide and requires a high number fungicide applications. The G143A substitution in inhibitor binding site cytochrome b V. confers level resistance to strobilurins targeting bc1 complex. aim this work was substitute labor intensive vitro assays, with faster quantitative PCR. An allele-specific qPCR method newly designed primer set successfully developed quantitatively determine frequency QoI-resistant A143 allele populations inaequalis. To be able suggest that molecular could applied as unique robust technique, we carried out sensitivity test trifloxystrobin; first testing relative germination subsequently confirmed quantification mutated frequencies on forty-nine Italian populations. gave similar pattern obtained using conidial predominantly sensitive resistant populations, variability between these two tests observed some heterogeneous assay study efficiently quantifies can conclude useful for at population fields, giving quick response also large amount samples.